All Plasmids – Hines Lab

ALL PLASMIDS

(as of 1/24/2015)

1. pLOX-TERT-iresTK; Insert:TERT, HSV-1 thymidine kinase (TERT, TP2, TRT, EST2, TCS1, hEST2, TK)
pHR:pHR; resistance: Ampicillin; created by:
2. pGL4.10-luc2; Insert:luc2 (Firefly Luciferase)
resistance: created by:
3. phRL-TK; Insert:RL (Renilla Luciferase)
resistance: created by:
4. pGEM T EZ; Insert:INSERT, No resistance: created by:
5. pLTR-RD114; Insert:glycoprotein, RD114 envelope pEGFP-C1 (Clontech) pEGFP-C1 (Clontech) resistance: Kanamycin; created by:
6. pSilencer-1.0-U6-needs-editing; resistance: created by:
7. RNAi pSiren RetroQ-needs editing; resistance: created by:
8. psPax (likely psPAX2) – 2nd Gen; resistance: AMP; created by:
9. pMD2.G (VSVG) – 2nd Gen; Insert:VSVG resistance: Amp; created by:
10. pmCherry-ATG5; Insert:(mouse), mCherry + ATG5 (Atg5, Apg5l, Atg5l, Paddy, C88337, AW319544, 2010107M05Rik, 3110067M24Rik
)
pEGFPC1 pEGFPC1 resistance: Kanamycin; Roberta Gottlieb, the fusion marker mCherry is from Roger Tsien at UCSD
Roberta Gottlieb, the fusion marker mCherry is from Roger Tsien at UCSD
created by:
11. CFP4 yPET; Insert:YPet pCEP4 pCEP4 resistance: Ampicillin; ?: ?; created by:
12. uses of GFP in transgenic vertebrates. (ed. article).
13. Plasmids ready for MAXI prep.
14. pBabe TERT Puro; resistance: created by:
15. CMV-LacZ AdenoX; resistance: created by:
16. pHIV-Wnt1HA; resistance: Varmus,H.E.
Fung,Y.K., Shackleford,G.M., Brown,A.M., Sanders,G.S. and Varmus,H.E. Nucleotide sequence and expression in vitro of cDNA derived from mRNA of int-1, a provirally activated mouse mammary oncogene. Mol. Cell. Biol. 5 (12), 3337-3344 (1985): Varmus,H.E.
Fung,Y.K., Shackleford,G.M., Brown,A.M., Sanders,G.S. and Varmus,H.E. Nucleotide sequence and expression in vitro of cDNA derived from mRNA of int-1, a provirally activated mouse mammary oncogene. Mol. Cell. Biol. 5 (12), 3337-3344 (1985); created by:
17. List of Plasmids (Yaswen Lab); resistance: created by:
18. Bissell Lab list of Plasmids Dec 2010; resistance: created by:
19. H2B-Orange resistance: AMP; created by:
20. H2B-Venus; resistance: AMP; created by:
21. pBabe puro-hTERT-HA; resistance: created by: Addgene
22. pLenti #43) pLenti6/CMV-H2b-mCherry (Blast); Insert:H2b-mCherry resistance: Ampicillin; created by: Curt Hines & Anya Afasizheva
23. pLenti #44) pLenti6/15ERE-H2b-EGFP (mCherry); Insert:H2b-EGFP , mCherry resistance: Ampicillin; created by: Curt Hines & Anya Afasizheva
24. pENTR D TOPO H2B-mCherry; Insert:H2B, Human histone, mCherry (H2B, Human histone fused to mCherry)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; PCR amplified from Addgene 20972: PCR amplified from Addgene 20972; created by: Curt Hines & Anya Afasizheva
25. pENTR D TOPO H2B-Orange; Insert:H2B, Human histone, Orange (H2B, Human histone fused to Orange)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; PCR amplified from Addgene 20969: PCR amplified from Addgene 20969; created by: Curt Hines & Anya Afasizheva
26. pENTR D TOPO H2B-Venus; Insert:H2B, Human histone, Venus (H2B, Human histone fused to Venus)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; PCR amplified from Addgene 20971: PCR amplified from Addgene 20971; created by: Curt Hines & Anya Afasizheva
27. pENTR D TOPO H2B-CFP; Insert:H2B, Human histone, CFP (H2B, Human histone fused to CFP)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; PCR amplified from Addgene 25998: PCR amplified from Addgene 25998; created by: Curt Hines & Anya Afasizheva
28. pENTR 5′ TOPO 15-ERE Promoter (not 25ERE); Insert:promoter, Estrogen response (15 Estrogen response Promoter)
pENTR 5′ TOPO:pENTR 5′ TOPO; resistance: Kan; Kornelia Polyak: Kornelia Polyak; created by: Curt Hines & Anya Afasizheva
29. pENTR 5′ TOPO 25-ERE Promoter Insert:promoter, Estrogen response (25 Estrogen response Promoter)
pENTR 5′ TOPO:pENTR 5′ TOPO; resistance: Kan; Kornelia Polyak: Kornelia Polyak; created by: Curt Hines & Anya Afasizheva
30. pENTR 5′ TOPO 25-ERE Promoter; Insert:promoter, Estrogen response (25 Estrogen response Promoter)
pENTR 5′ TOPO:pENTR 5′ TOPO; resistance: Kan; Kornelia Polyak: Kornelia Polyak; created by: Curt Hines & Anya Afasizheva
31. pLenti #45) pLenti6/CMV-H2b-Venus (blast); Insert:H2b-Venus resistance: Ampicillin; created by: Curt Hines & Anya Afasizheva
32. pLenti #46) pLenti6/CMV-H2b-mOrange (blast); Insert:H2b-mOrange resistance: Ampicillin; created by: Curt Hines & Anya Afasizheva
33. pLenti #47) pLenti6/CMV-ZsYellow (blast); Insert:ZsYellow resistance: Ampicillin; created by: Curt Hines & Anya Afasizheva
34. pLenti #48) pLenti6/CMV-Cerulean[blast]; Insert:Cerulean pLENTI/6:pLENTI/6; resistance: Ampicillin; created by: Curt Hines & Anya Afasizheva
35. pENTR D TOPO E6; Insert:E6, HPV-16 pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines & Anya Afasizheva
36. pENTR D TOPO E6/E7; Insert:E6/E7, HPV-16 pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines & Anya Afasizheva
37. pENTR D TOPO 5’miR|XhoI/EcoRI|3’miR; Insert:5’miR|XhoI/EcoRI|3’miR pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines & Anya Afasizheva
38. pENTR D TOPO GFP 5’miR|XhoI/EcoRI|3’miR; Insert:5’miR|XhoI/EcoRI|3’miR pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines & Anya Afasizheva
39. pLenti #49) pLenti6/CMV-GSE22 (Citrine); Insert:Citrine, GSE22 (inhibits p53) and pLENTI/6:pLENTI/6; resistance: Ampicillin; created by: Curt Hines & Anya Afasizheva
40. pLenti #50) pLenti6/CMV-HPV16 E6/E7 (Citrine); Insert:E6/E7, HPV16 pLENTI/6:pLENTI/6; resistance: Ampicillin; PCR cloned from p1321 HPV-16 E6/E7 (peter howley): PCR cloned from p1321 HPV-16 E6/E7 (peter howley); created by: Curt Hines & Anya Afasizheva
41. pLenti #53) pLenti6/CMV-HER2 CA (V659E) Version #002 (Citrine); Insert:#002, HER2 CA (V659E) Version pLENTI/6:pLENTI/6; resistance: Ampicillin; LR recombination with pENTR 5′ TOPO CMVpro, pENTR D TOPO HER2 CA (V659E) Version #002, pLenti6/R4R2/v5-DEST (Citrine): LR recombination with pENTR 5′ TOPO CMVpro, pENTR D TOPO HER2 CA (V659E) Version #002, pLenti6/R4R2/v5-DEST (Citrine); created by: Curt Hines & Anya Afasizheva
42. pLenti #51) pLenti6/CMV-HPV16 E6 (Citrine); Insert:E6, HPV16 pLENTI/6:pLENTI/6; resistance: Ampicillin; LR recombination with pENTR 5′ TOPO CMVpro, pENTR D TOPO E6, pLenti6/R4R2/v5-DEST (Citrine): LR recombination with pENTR 5′ TOPO CMVpro, pENTR D TOPO E6, pLenti6/R4R2/v5-DEST (Citrine); created by: Curt Hines & Anya Afasizheva
43. pLenti #52) pLenti6/CMV-HPV16 E7 (Citrine); Insert:E7, HPV16 pLENTI/6:pLENTI/6; resistance: Ampicillin; LR recombination with pENTR 5′ TOPO CMVpro, pENTR D TOPO E7, pLenti6/R4R2/v5-DEST (Citrine): LR recombination with pENTR 5′ TOPO CMVpro, pENTR D TOPO E7, pLenti6/R4R2/v5-DEST (Citrine); created by: Curt Hines & Anya Afasizheva
44. pLenti #57) pLenti6/TRE-tight/ZsGreen (rtTA3); Insert:ZsGreen, TREtight & resistance: Ampicillin; created by: Curt Hines and Anya Afasizheva
45. LXSN GFP; Insert:GFP resistance: created by: Alvin_Lo_and_Alain_B.
46. MSCV GFP; Insert:GFP resistance: AMP; created by: Alvin_Lo_and_Alain_B.
47. pENTR1A DS-SATB1; resistance: Kan; created by: Michael Balamotis/LBNL
48. H2B-mCherry; Insert:H2b-mCherry (Histone cluster 1, H2bb, H2b.1,HIST1H2BB, H2B.1, H2B/f, H2BFF, MGC119804)
pcDNA3:pcDNA3; resistance: Amp; created by: Robert Benezra
49. EGFP-N-BORIS; resistance: created by: Benhattar
50. CMV6-xL4-BORIS; resistance: created by: Benhattar
51. pENTR1A SV40 small + Large T antigen; Insert:antigen, SV40 small + Large T (SV40 t+T Ag)
pENTR1A:pENTR1A; resistance: Kan; small + Large T Ag DNA was obtained from Dr. Stephane Gobeil.: small + Large T Ag DNA was obtained from Dr. Stephane Gobeil.; created by: Eric Campeau
52. GSE22 T329A in pENTR1A (w187-1) Insert:Genetic suppressor element #22, T329A (GSE22, T329A (R. norvegicus (rat)))
2277:2277; resistance: Kanamycin; GSE22 was obtained from Dr.Christian Beausejour: GSE22 was obtained from Dr.Christian Beausejour; created by: Eric Campeau
53. pLenti CMV RasV12 Neo (w108-1) Insert:G12V, HRAS (HRAS, CTLO, HAMSV, HRAS1, K-RAS, N-RAS, RASH1, C-H-RAS, H-RASIDX, C-BAS/HAS, C-HA-RAS1)
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; resistance: Ampicillin; RasV12 cDNA obtained from Dr. Christian Beausejour.: RasV12 cDNA obtained from Dr. Christian Beausejour.; created by: Eric Campeau
54. pTRE-Tight caspase-3 (p12)::nz [TU#817]
Insert:caspase-3 (p12) ::nz, from caspase-3 from MGC full length cDNA clone ID 4419175. (caspase-3, CASP3, CPP32, SCA-1, CPP32B)
pTRE-Tight:pTRE-Tight; resistance: AMP; created by: Martin Chalfie
55. pIntron Sindbis; Insert:UCLA phCMV-g

described in:
A general method for the generation of high-titer, pantropic retroviral vectors: highly efficient infection of primary hepatocytes. Yee, et.al.
PNAS September 27, 1994 vol. 91 no. 20 9564-9568 resistance: AMP; created by: Irvin Chen
57. pCMV-DsRed-Express; Insert:DsRED-Express resistance: created by: Clontech
58. pDsRED Express DR; Insert:DR, DsRED Express resistance: created by: Clontech
59. pZsGreen-DR; resistance: created by: Clontech
60. pZsYELLOW1-N1; Insert:ZsYellow resistance: created by: Clontech
61. pREV Tet ON; resistance: created by: Clontech
62. pRevTRE; resistance: created by: Clontech
63. pEGFP-1; resistance: created by: Clontech
64. pTRE-TIGHT (info only, we don’t have this); resistance: AMP; created by: Clontech
65. EGFP-N1; resistance: created by: Clontech
66. pShuttle; Insert:Empty resistance: Kanamycin; created by: Clontech
67. pShuttle-Lacz; Insert:lacZ resistance: Kanamycin; created by: Clontech
68. pLenti #60) pLenti6/CMV-EGFP-HP1a; Insert:EGFP-HP1a resistance: Ampicillin; created by: Kremena Curt Hines, Maria
69. resistance: created by: Curt_&_IDT
70. pAAV-pLenti Polylinker; resistance: AMP; created by: Curt_Hines
71. TERT-Pro-EGFP AdenoX resistance: created by: Curt_Hines
72. CMV-Pro-EGFP AdenoX resistance: created by: Curt_Hines
73. GS3955 AdenoX resistance: created by: Curt_Hines
74. CMV-Pro-DsRED Express AdenoX resistance: created by: Curt_Hines
75. No-Pro-EGFP AdenoX resistance: created by: Curt_Hines
76. pLenti6/R4R2/v5-DEST (Cerulean); resistance: Ampicillin; created by: Curt_Hines
77. pLenti6/R4R2/v5-DEST (EGFP); resistance: Ampicillin; created by: Curt_Hines
78. pLenti6/R4R2/v5-DEST (mCherry); resistance: Ampicillin; created by: Curt_Hines
79. pLenti6/R4R2/v5-DEST (Citrine); resistance: Ampicillin; created by: Curt_Hines
80. pLenti6/R4R2/v5-DEST (NEO); resistance: Ampicillin; created by: Curt_Hines
81. pLenti6/R4R2/v5-DEST (Luc2); resistance: Ampicillin; created by: Curt_Hines
82. pLenti6/R4R2/v5-DEST (DsRED Express); resistance: Ampicillin; created by: Curt_Hines
83. pLenti6/R4R2/v5-DEST (ZsGreen); resistance: Ampicillin; created by: Curt_Hines
84. pLenti6/R4R2/v5-DEST (hRASV12); resistance: Ampicillin; created by: Curt_Hines
85. pENTR221 hRASV12; Insert:hRASV12 (hRASG12V, v-Ha-ras Harvey rat sarcoma viral oncogene, variant 3)
resistance: BP reaction from Eric Campeau’s :pLenti CMV RasV12 Neo (w108-1) BP reaction from Eric Campeau’s :pLenti CMV RasV12 Neo (w108-1) created by: Curt_Hines
86. pLenti6/R4R2/v5-DEST (rtTA3); Insert:Advanced, rtTA3 – Tet On resistance: Ampicillin; created by: Curt_Hines&Anya
87. pGEMTez-R4-cm-ccdb-R2 DEST; Insert:construct, multisite gateway R4-R2 pGEMTEZ:pGEMTEZ; resistance: Amp; created by: Curt_Hines/Melody_Ju
88. pENTR 1A (L1/L2) Brainbow 1.0L; resistance: created by: Doug
89. pENTR CRE; resistance: created by: doug
90. LV-CFP (pLKO.1 H2b-CFP); pLKO.1:pLKO.1; resistance: Amp; created by: Elaine_Fuchs
91. aaaa-LIST OF PLASMIDS TO ENTER; resistance: created by: aaaa-LIST OF PLASMIDS TO ENTER
92. H2B-HcRed; Insert:H2B-HcRed (H2B-HcRed, Human Histone H2B -Heteractis crispa red fluorescent protein)
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; resistance: Ampicillin; created by: Eric_Campeau
93. pLenti CMV rtTA3 Blast (w756-1); Insert:mutant, Tetracycline repressor A3 (rtTA3, Tet-on advanced, TETon advanced)
p156RRL-sinPPT-CMV-GFP-PRE/Nhe I:p156RRL-sinPPT-CMV-GFP-PRE/Nhe I; resistance: Amp; Campeau notes that The rtTA3 element was a gift from Dr. Dominic Esposito.: Campeau notes that The rtTA3 element was a gift from Dr. Dominic Esposito.; created by: Eric_Campeau
94. E7 in pENTR1A (w186-1) Insert:E7, Human papilloma virus pENTR1A:pENTR1A; resistance: Kanamycin E7 cDNA was obtained from Dr. Christian Beausejour: E7 cDNA was obtained from Dr. Christian Beausejour; created by: Eric_Campeau
95. pLenti6/CMV-hRAS V12 (Neo) Addgene22259; Insert:V12, hRAS resistance: Ampicillin; created by: Eric_Campeau
96. pLENTI CMVTetO -PURO DEST R1R2; pLENTI:pLENTI; resistance: AMP; created by: Eric_Campeau
97. pENTR/pSM2(CMV) GFP (w513-1) Insert:(SM2), shRNA miR cloning site pENTR1A:pENTR1A; resistance: Kan; created by: Eric_Campeau
98. hTERT Plasmid (hTERT qPCR Std); Insert:(234bp), partial coding seq of hTERT pGEM T EZ pGEM T EZ resistance: Amp; created by: Curt Hines
99. TBP Plasmid (hTERT qPCR Std); Insert:(89bp), partial coding seq of TBP pGEM T EZ pGEM T EZ resistance: Amp; created by: Curt Hines
100. pShuttle-DsREDExpress; Insert:DsRED-Express resistance: Kanamycin; pCMV-DsREDExpress (from Clontech): pCMV-DsREDExpress (from Clontech); created by: Curt Hines
101. pShuttle-CMVdeleted; Insert:Empty resistance: Kanamycin; created by: Curt Hines
102. pShuttle-EGFP (CMV-EGFP); Insert:EGFP pShuttle parental:pShuttle parental; resistance: Kanamycin; pEGFP-1: pEGFP-1; created by: Curt Hines
103. pShuttle-hTERTpro-EGFP; Insert:hTERTPromoter-EGFP pShuttle CMV deleted:pShuttle CMV deleted; resistance: Kanamycin; pEGFP-1: pEGFP-1; created by: Curt Hines
104. pShuttle-CMVdel-EGFP; Insert:Promoterless-EGFP pShuttle CMV deleted:pShuttle CMV deleted; resistance: Kanamycin; pEGFP-1: pEGFP-1; created by: Curt Hines
105. pshuttle cmv del; Insert:Empty resistance: Kanamycin; created by: Curt Hines
106. pShuttle-GS3955-EGFP; Insert:Empty (GS3955, EGFP)
resistance: Kanamycin; created by: Curt Hines
107. pShuttle-HA-BORIS; Insert:Empty resistance: Kanamycin; created by: Curt Hines
108. pENTR D TOPO EGFP; Insert:EGFP (EGFP, GFP)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines
109. pLenti #1) pLenti6/UbC-DsRedExpress-DR (blast); Insert:UbC Promoter, DsRedExpress-DR (destabalized) (Destabalized DsRedExpress)
resistance: Ampicillin; created by: Curt Hines
110. pLenti #2) pLenti6/UbC-ZsGreen-DR (blast); Insert:UbC Promoter, ZsGreen-DR (destabalized) (Destabalized ZsGreen)
resistance: Ampicillin; created by: Curt Hines
111. pLenti #3) pLenti6/UbC-ZsYellow (blast); Insert:UbC Promoter, ZsYellow resistance: Ampicillin; created by: Curt Hines
112. pLenti #4) pLenti6/UbC-Neo (blast); Insert:UbC Promoter, Neo resistance: Ampicillin; PCR amplification of Neo from pREV TET-ON
PCR amplification of Neo from pREV TET-ON
created by: Curt Hines
113. pLenti #5) pLenti6/UbC-Puro (blast); Insert:UbC Promoter, Puro resistance: Ampicillin; PCR amplification of Puro from pLenti-TetO-Puro
PCR amplification of Puro from pLenti-TetO-Puro
created by: Curt Hines
114. pLenti #15) pLenti6/K19pro-DsRedExpress (blast); Insert:Keratin 19 Promoter, DsRedExpress-DR resistance: Ampicillin; created by: Curt Hines
115. pLenti #14) pLenti6/K19pro-EGFP (blast); Insert:Keratin 19 Promoter, EGFP resistance: Ampicillin; created by: Curt Hines
116. pLenti #8) pLenti6/K19Pro-Puro (blast); Insert:K19 Promoter, Puro resistance: Ampicillin; PCR amplification of Puro from pLenti-TetO-Puro
PCR amplification of Puro from pLenti-TetO-Puro
created by: Curt Hines
117. pLenti #9) pLenti6/K14pro-DsRedExpress-DR (blast); Insert:Keratin 14 Promoter, DsRedExpress-DR (destabalized) (Destabalized DsRedExpress)
resistance: Ampicillin; created by: Curt Hines
118. pLenti #11) pLenti6/K14Pro-Neo (blast); Insert:K14 Promoter, Neo resistance: Ampicillin; PCR amplification of Puro from pLenti-TetO-Puro
PCR amplification of Puro from pLenti-TetO-Puro
created by: Curt Hines
119. pLenti #10 pLenti6/K14pro-ZsGreen-DR (blast); Insert:Keratin 14 Promoter, DsRedExpress-DR (destabalized) (Destabalized DsRedExpress)
resistance: Ampicillin; created by: Curt Hines
120. pLenti #6) pLenti6/K19pro-DsRedExpress-DR (blast); Insert:Keratin 19 Promoter, DsRedExpress-DR (destabalized) (Destabalized DsRedExpress)
resistance: Ampicillin; created by: Curt Hines
121. pLenti #7) pLenti6/K19pro-ZsGreen-DR (blast); Insert:Keratin 19 Promoter, ZsGreen-DR (destabalized) (Destabalized DsRedExpress)
resistance: Ampicillin; created by: Curt Hines
122. pENTR D TOPO mCherry; Insert:mCherry (RFP)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; PCR/TOPO cloned from pmCherry ATG5: PCR/TOPO cloned from pmCherry ATG5; created by: Curt Hines
123. pENTR D TOPO Cerulean; Insert:cerulean (CFP)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; PCR/TOPO cloned from Cerulean: PCR/TOPO cloned from Cerulean; created by: Curt Hines
124. pENTR D TOPO ZsGreen; Insert:ZsGreen (GFP)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; PCR/TOPO cloned from Cerulean: PCR/TOPO cloned from Cerulean; created by: Curt Hines
125. pENTR D TOPO ZsGreen; Insert:ZsGreen pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines
126. pENTR D TOPO yPET; Insert:yPET (YFP)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines
127. pENTR D TOPO luc2; Insert:luc2 (Firefly Luciferase)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines
128. pENTR D TOPO RL Luciferase; Insert:RL (Renilla Luciferase)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines
129. pENTR D TOPO PURO; Insert:PURO (Puromycin Resistance)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines
130. pENTR D TOPO NEO; Insert:NEO (Neomycin Resistance)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines
131. pENTR D TOPO ZsYellow; Insert:ZsYellow (YFP)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines
132. pENTR D TOPO DsRed Express; Insert:Express, DsRed (RFP)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines
133. pENTR D TOPO DsRed Express-DR; Insert:(destabalized), DsRed Express-DR (RFP)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines
134. pENTR D TOPO ZsGreen-DR; Insert:(destabalized), ZsGreen-DR pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines
135. pENTR 5′ TOPO K19pro; Insert:Promoter, Keratin 19 pENTR 5′ TOPO:pENTR 5′ TOPO; resistance: Kan; created by: Curt Hines
136. pENTR 5′ TOPO K14pro; Insert:Promoter, Keratin 14 pENTR 5′ TOPO:pENTR 5′ TOPO; resistance: Kan; created by: Curt Hines
137. pENTR 5′ TOPO HSV-TKpro; Insert:Promoter, Keratin 14 pENTR 5′ TOPO:pENTR 5′ TOPO; resistance: Kan; created by: Curt Hines
138. pENTR 5′ TOPO CMVpro; Insert:Promoter, CMV pENTR 5′ TOPO:pENTR 5′ TOPO; resistance: Kan; created by: Curt Hines
139. pLenti #13) pLenti6/UbC-DsRed Express (blast); Insert:UbC Promoter, DsRedExpress resistance: Ampicillin; created by: Curt Hines
140. pLenti #16) pLenti6/K14Pro-EGFP (blast); Insert:K14 Promoter, EGFP resistance: Ampicillin; created by: Curt Hines
141. pLenti #17) pLenti6/K14Pro-DsRed Express (blast); Insert:K14 Promoter, DsRed Express resistance: Ampicillin; created by: Curt Hines
142. pLenti #12) pLenti6/UbC-EGFP Insert:UbC Promoter, EGFP resistance: Ampicillin; created by: Curt Hines
143. pLenti #26??XX) pLenti6/K14Pro-ZsGreen; Insert:K14Pro-ZsGreen resistance: Ampicillin; created by: Curt Hines
144. pLenti #21) pLenti6/CMV-EGFP (blast); Insert:CMV Promoter, EGFP (Enhanced Green Fluorescent Protein)
resistance: Ampicillin; created by: Curt Hines
145. pLenti #18) pLenti6/UbC-Cerulean (blast); Insert:UbC Promoter, Cerulean (CFP variant)
resistance: Ampicillin; created by: Curt Hines
146. pLenti #19) pLenti6/UbC-ZsGreen (blast); Insert:UbC Promoter, ZsGreen resistance: Ampicillin; created by: Curt Hines
147. pLenti #20) pLenti6/UbC-mCherry (blast); Insert:UbC Promoter, mCherry resistance: Ampicillin; created by: Curt Hines
148. pLenti #22) pLenti6/CMV-ZsGreen (blast); Insert:CMV Promoter, ZsGreen (GFP)
resistance: Ampicillin; created by: Curt Hines
149. pLenti #23) pLenti6/K19pro-ZsGreen (blast); Insert:Keratin 19 Promoter, ZsGreen resistance: Ampicillin; created by: Curt Hines
150. pLenti #24) pLenti6/K14Pro-ZsGreen (blast); Insert:K14Pro-ZsGreen (GFP, ZsGreen, EGFP, Green)
resistance: Ampicillin; created by: Curt Hines
151. pLenti #18) pLenti6/UbC-Cerulean; Insert:UbC Promoter, Cerulean (CFP variant)
resistance: Ampicillin; created by: Curt Hines
152. pENTR D TOPO HSV Thymidine Kinase; Insert:Thymidine kinase; tk, HSVTK pENTR D TOPO:pENTR D TOPO; resistance: Kan; PCR: PCR; created by: Curt Hines
153. pLenti #25) pLenti6/K14Pro-Thymidine Kinase (blast); Insert:K14Pro-TK (HSV-TK, Thymidine Kinase)
resistance: Ampicillin; created by: Curt Hines
154. pLenti #26) pLenti6/CMVPro-Thymidine Kinase (blast); Insert:CMVpro-TK (Thymidine Kinase, HSV-TK)
resistance: Ampicillin; created by: Curt Hines
155. pENTR 5′ TOPO TRE-TIGHTpro; Insert:TRE-TIGHT (modified Tet response element)
pENTR 5′ TOPO:pENTR 5′ TOPO; resistance: Kan; created by: Curt Hines
156. pENTR D TOPO H2B-EGFP; Insert:H2B, Human histone, EGFP (H2B, Human histone fused to EGFP, GFP, )
pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Curt Hines
157. pLenti #27) pLenti6/TRE-TIGHT-ZsGreen (blast); Insert:TRE_TIGHT-ZsGreen resistance: Ampicillin; created by: Curt Hines
158. pLenti #28) pLenti6/CMVPro-H2B-EGFP (Blast); Insert:CMVPro-H2B-EGFP resistance: Ampicillin; created by: Curt Hines
159. pLenti #29) pLenti6/TRE-TIGHT-Cre Recombinase (blast); resistance: Ampicillin; created by: Curt Hines
160. pLenti #30) pLenti6/CMVPro-Brainbow 1.0L (blast); Insert:EYFP, dTomato ; mCerulean ; resistance: Ampicillin; created by: Curt Hines
161. pLenti #28) pLenti6/CMVPro-H2B-EGFP; Insert:CMVPro-H2B-EGFP resistance: Ampicillin; created by: Curt Hines
162. pLenti #99) pLenti6/XX; Insert:CA, TREtight-Her2 resistance: Ampicillin; created by: Curt Hines
163. pLenti #100) pLenti6/XX; Insert:CA, TREtight-Her2 resistance: Ampicillin; created by: Curt Hines
164. pENTR 5′ TOPO BclI-EGFP -Acc65I; Insert:EGFP, BclI/Acc65I tailed attL4-GENE-attR1:attL4-GENE-attR1; resistance: Kan; created by: Curt Hines
165. pENTR 5′ TOPO BclI-Cerulean -Acc65I; Insert:Cerulean, BclI/Acc65I tailed pENTR 5′ TOPO:pENTR 5′ TOPO; resistance: Kan; created by: Curt Hines
166. pENTR 5′ TOPO BclI-Citrine -Acc65I; Insert:Citrine, BclI/Acc65I tailed attL4-GENE-attR1:attL4-GENE-attR1; resistance: Kan; created by: Curt Hines
167. pENTR 5′ TOPO BclI-mCherry -Acc65I; Insert:mCherry, BclI/Acc65I tailed attL4-GENE-attR1:attL4-GENE-attR1; resistance: Kan; created by: Curt Hines
168. pENTR 5′ TOPO BclI-NEO* (1SNP) -Acc65I; Insert:NEO(*1SNP), BclI/Acc65I tailed attL4-GENE-attR1:attL4-GENE-attR1; resistance: Kan; created by: Curt Hines
169. pENTR 5′ TOPO EF1 alpha Promoter; Insert:Promoter, EF1 alpha attL4-GENE-attR1:attL4-GENE-attR1; resistance: Kan; created by: Curt Hines
170. pENTR D TOPO HER2 CA (V659E, I654V, I655V) Version #001
(Note this was called V654E by Addgene depositor, but it should be V659E..there isnt a V654..there is an I654V); Insert:HER2, Constitutively Active (ERBB2, NEU, NGL, HER2, TKR1, CD340, HER-2, MLN 19, HER-2/neu)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; PCR cloned from Addgene# 16259, Mien-Chie Hung lab: PCR cloned from Addgene# 16259, Mien-Chie Hung lab; created by: Curt Hines
171. pLenti #41) pLenti6/CMV-Luc2 (Blast) Insert:Luc2 (Firefly luciferase)
resistance: Ampicillin; created by: Curt Hines
172. pLenti #42) pLenti6/CMV-Luc2 (mCherry) Insert:Luc2, mCherry (Firefly luciferase, mCherry)
resistance: Ampicillin; created by: Curt Hines
173. pLenti #31) pLenti6/TRE-tight/Her2-CA(Blast); Insert:CA, TREtight-Her2 resistance: Ampicillin; created by: Curt Hines
174. pLenti #32) pLenti6/TRE-tight/Her2-CA(ZsGreen); Insert:ZsGreen, TREtight-Her2 CA & resistance: Ampicillin; created by: Curt Hines
175. pLenti #33) pLenti6/CMV-H2b-GFP (mCherry); Insert:mCherry, CMV-H2b-GFP & resistance: Ampicillin; created by: Curt Hines
176. pLenti #34) pLenti6/CMV-Cerulean (DsRed Express); Insert:Express, Cerulean & DsRed resistance: Ampicillin; created by: Curt Hines
177. pLenti #35) pLenti6/CMV-Cerulean (Citrine); Insert:Citrine, Cerulean & resistance: Ampicillin; created by: Curt Hines
178. pLenti #36) pLenti6/CMV-hRAS V12 (Cerulean); Insert:Cerulean, hRAS V12 & resistance: Ampicillin; created by: Curt Hines
179. pLenti #37) pLenti6/UbC-hRAS V12 (Cerulean); Insert:Cerulean, hRAS V12 & resistance: Ampicillin; created by: Curt Hines
180. pLenti #38) pLenti6/CMV-SV40 small and Large T (DsRed Express); Insert:Express, SV40 small and Large T & DsRed resistance: Ampicillin; created by: Curt Hines
181. pLenti #39) pLenti6/UbC-SV40 small and Large T (DsRed Express); Insert:Express, SV40 small and Large T & DsRed resistance: Ampicillin; created by: Curt Hines
182. pLenti #40) pLenti6/CMV-SV40 small and Large T (Citrine); Insert:Citrine, SV40 small and Large T & resistance: Ampicillin; created by: Curt Hines
183. pENTR D TOPO HER2 CA (V659E) Version #002
(Note this was called V654E by Addgene depositor, but it should be V659E..there isnt a V654..there is an I654V)

Also, some also found the mutations we saw in the first plasmid (version #1) we received from Addgene. Addgene contacted the authors and verified that version #1 was incorrect. They sent us a replacement, and we re-cloned into pENTR DTOPO

Insert:HER2, Constitutively Active (ERBB2, NEU, NGL, HER2, TKR1, CD340, HER-2, MLN 19, HER-2/neu)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; PCR cloned from Addgene# 16259 replacement, Mien-Chie Hung lab: PCR cloned from Addgene# 16259 replacement, Mien-Chie Hung lab; created by: Curt Hines
184. pLenti #59) pLenti6/CMV-GFP-53bp1 (Blast); Insert:fusion, GFP-53BP1 resistance: Ampicillin; created by: Curt Hines
185. pLenti #58) pLenti6/CMV-mCherry-53bp1 (Blast); Insert:fusion, mCherry-53BP1 resistance: Ampicillin; created by: Curt Hines
186. pLenti #54) pLenti6/CMV-mSATB1 (EGFP); resistance: Ampicillin; created by: Curt Hines
187. pLenti #55) pLenti6/UbC-mSATB1 (EGFP); resistance: Ampicillin; created by: Curt Hines
188. pLenti #56) pLenti6/CMV-mSATB1 (Blast); resistance: Ampicillin; created by: Curt Hines
189. pENTR 5′ TOPO rtTA3; Insert:on, rtTA3 tet pENTR 5′ TOPO:pENTR 5′ TOPO; resistance: Kan; created by: Curt Hines&Anya
190. A. Hiragun, Y. Yoshida, M. Sato, T. Tominaga & H. Mitsui. Isolation of two syngeneic cell lines from a rat mammary carcinoma: growth factor production by neoplastic epithelial cells. J Natl Cancer Inst 75, 471-482 (1985).
191. p1321 HPV-16 E6/E7 Insert:E6/E7, HPV-16 (HPV-16 E6
HPV-16 E7 )
p1318:p1318; resistance: Ampicillin created by: Peter Howley
192. Cre Stoplight; resistance: created by: Hughes
193. HER2 CA (V654E) ???
(I Believe this should be V659E..there isnt a V654); Insert:HER2, Constitutively Active (ERBB2, NEU, NGL, HER2, TKR1, CD340, HER-2, MLN 19, HER-2/neu)
pcDNA3:pcDNA3; resistance: Ampicillin; created by: Mien-Chie Hung
194. IDT. DNA Calculator. in IDT melting temperature reverse complement DNA (ed. file, XML).
195. pCSC-SP-PW-Nep (aka: pBOB-NEP); Insert:CD10, Neprilysin (MME (NEP, SFE, CD10, CALLA, MGC126681, MGC126707, DKFZp686O16152))
pBOB:pBOB; resistance: Amp; created by: Inder_Verma
196. I. Inoue, I. Tsutsui & Q. Bone. Long-lasting potassium channel inactivation in myoepithelial fibres is related to characteristics of swimming in diphyid siphonophores. J Exp Biol 208, 4577-4584 (2005).
197. pLP1; Insert:gag/pol resistance: Ampicillin; created by: INVITROGEN
198. pLP2; Insert:rev resistance: Ampicillin; created by: INVITROGEN
199. pLP/VSVG; Insert:VSVG resistance: Ampicillin; created by: INVITROGEN
200. pLenti6/R4R2/v5-DEST (Blasticidin); resistance: Ampicillin; created by: Invitrogen
201. pENTR 5′ TOPO UbCpro; Insert:Promoter, UbC pENTR 5′ TOPO:pENTR 5′ TOPO; resistance: Kan; created by: INVITROGEN
202. pENTR1A; pENTR1A pENTR 1A:pENTR1A pENTR 1A; resistance: created by: INVITROGEN
203. pLenti6/UbC/V5-GW/lacZ; resistance: created by: Invitrogen
204. pDONR 221; Insert:ccdb, cm resistance: Kan (and chloramphenicol); created by: INVITROGEN
205. pENTR GUS; resistance: created by: Invitrogen
206. pENTR1A Dual Selection Vector; resistance: Kan; created by: Invitrogen
207. pEGFP-VSVG; Insert:pEGFP-VSVG pEGFP-N1, Clontech:pEGFP-N1, Clontech; resistance: Kan; created by: Jennifer_Lippincott-Schwartz_Lab
208. GP64; Insert:protein, baculovirus GP64 envelope resistance: created by: Dr. Joshua_Zimmerberg
209. pShuttle CMVdel XbaI delta; Insert:None pShuttle:pShuttle; resistance: Kan created by: Curt Hines and Melody Ju
210. pShuttle CMVdel XbaI delta USER; Insert:None pShuttle:pShuttle; resistance: Kan created by: Curt Hines and Melody Ju
211. pShuttle CMVdel XbaI delta USER R2R4; Insert:None pShuttle:pShuttle; resistance: Kan created by: Curt Hines and Melody Ju
212. pShuttle-CMVdel-R4-R2dest; Insert:Empty resistance: Kanamycin; created by: Curt Hines and Melody Ju
213. pENTR TOPO EGFP-HP1a; Insert:EGFP-HP1a (EGFP, GFP)
pENTR D TOPO:pENTR D TOPO; resistance: Kan; created by: Costes Lab
214. pShuttle 25ERE-GFP; Insert:25ERE-GFP resistance: created by: Polyak Lab
215. pENTR1A-GFP-53bp1; Insert:fusion, GFP-53bp1 pENTR1A pENTR 1A ???? (e-mail says pENTR1A dual selection):pENTR1A pENTR 1A ???? (e-mail says pENTR1A dual selection); resistance: KAN; created by: Yeuyong-Costes Lab
216. pENTR1A-mcherry-53bp1; Insert:fusion, GFP-53bp1 pENTR1A pENTR 1A ???? (e-mail says pENTR1A dual selection):pENTR1A pENTR 1A ???? (e-mail says pENTR1A dual selection); resistance: KAN; created by: Yeuyong-Costes Lab
217. pBig-HA-BORIS; resistance: created by: Lobanenkov
218. pBig2 empty vector resistance: created by: Lobanenkov
219. pCAG myc-p160 ROCK KD-IA; Insert:kinase defective, Rho-binding, dominant negative mutant resistance: created by: Shuh Narumiya
220. Mother Nature. Genetic Code.
221. Muc1, Human MGC Verified FL cDNA (IRCM); Insert:MUC1 pCR-BluntII-TOPO pCR-BluntII-TOPO resistance: created by: open_Biosystems
222. pCMV-Sport6 P-Selectin; pCMV-Sport6:pCMV-Sport6; resistance: AMP; created by: Open_Biosystems
223. Cerulean; Insert:Cerulean, monomeric (mCER, CFP-like)
pEGFP-C1:pEGFP-C1; resistance: Kanamycin; synthetic: synthetic; created by: Piston
224. pENTR1A-IRES2-EGFP
pENTR1A:pENTR1A; resistance: Kan; created by: Acquired from Francis Rodier
225. pSub201; resistance: created by: Samulski
226. Blank; resistance: created by: Samulski
227. pscAAV2-CMV-GFP_or_pHpa-trs-sk; resistance: created by: Samulski
228. pXR6; resistance: AMP; created by: Samulski
229. pXR5; resistance: AMP; created by: Samulski
230. pXR4; resistance: AMP; created by: Samulski
231. pXR3; resistance: AMP; created by: Samulski
232. pXR2; resistance: AMP; created by: Samulski
233. pXR1; resistance: AMP; created by: Samulski
234. XX6-80; resistance: created by: Samulski
235. XX2; resistance: created by: Samulski
236. pXR5-Bam resistance: AMP; created by: Samulski
237. EBO deltaO (pEZGP 309-489); Insert:(GP), Ebola Zaire strain DeltaO mutant – Envelope protein pcDNA3.1 (Invitrogen, Carlsbad, Calif.)-derived plasmids (cat#V790-20):pcDNA3.1 (Invitrogen, Carlsbad, Calif.)-derived plasmids (cat#V790-20); resistance: Ampicilin; created by: David Sanders
238. EBO (pEZGP 309-489); Insert:(GP), Ebola Zaire strain Envelope protein pcDNA3.1 (Invitrogen, Carlsbad, Calif.)-derived plasmids (cat#V790-20):pcDNA3.1 (Invitrogen, Carlsbad, Calif.)-derived plasmids (cat#V790-20); resistance: Ampicilin; created by: David Sanders
239. rAAV6 Y445F; resistance: AMP; created by: David Schaffer
240. VSVG C30; resistance: AMP; created by: David Schaffer
241. VSVG C48; resistance: AMP; created by: David Schaffer
242. VSVG C52; resistance: AMP; created by: David Schaffer
243. VSVG C173; resistance: AMP; created by: David Schaffer
244. pHCMV-AmphoEnv; Insert:gene, 4070A Amphotropic Murine leukemia virus envelope pHMCV (Clontech):pHMCV (Clontech); resistance: Ampicillin; created by: Sena-Esteves
245. pHCMV-LCMV-WE; Insert:gene, Lymphocytic choriomeningitis virus glycoprotein LCMVGP pHMCV (Clontech):pHMCV (Clontech); resistance: Ampicillin; created by: Sena-Esteves
246. pHCMV-10A1; Insert:mRNA, 10A1 Murine leukemia virus envelope pHMCV (Clontech):pHMCV (Clontech); resistance: Ampicillin; created by: Sena-Esteves
247. pHCMV-LCMV-Arm53b; Insert:glycoprotein, LCMVArm53b envelope pHMCV (Clontech):pHMCV (Clontech); resistance: Ampicillin; created by: Sena-Esteves
248. pHCMV-MokolaG; Insert:gene, Mokola virus glycoprotein (G) pHMCV (Clontech):pHMCV (Clontech); resistance: Ampicillin; The gene was obtained from a plasmid provided by Dr. Jacob Reiser.: The gene was obtained from a plasmid provided by Dr. Jacob Reiser.; created by: Sena-Esteves
249. pHCMV-RabiesG; Insert:protein, Rabies virus G (RABVgp4)
pHMCV (Clontech):pHMCV (Clontech); resistance: Ampicillin; The gene was obtained from a plasmid provided by Dr. Jacob Reiser.: The gene was obtained from a plasmid provided by Dr. Jacob Reiser.; created by: Sena-Esteves
250. pCMV-MCS resistance: AMP; created by: Stratagene
251. pAAV-LacZ; resistance: AMP; created by: Stratagene
252. pAAV-RC (rep2-cap2); resistance: AMP; created by: Stratagene
253. pHelper (AAV); resistance: AMP; created by: Stratagene
254. pAAV-MCS; resistance: AMP; created by: Stratagene
255. pAAV-hrGFP; resistance: AMP; created by: Stratagene
256. pAAV-IRES-hrGFP; resistance: AMP; created by: Stratagene
257. pCMV-MCS + pLENTI polylinker; resistance: AMP; created by: Stratagene
258. YFP-PBD (Citrine tag); Insert:CITRINE (YFP), p21-activated kinase 1 PBD, PAK1 (PAK1, PAKalpha, MGC130000, MGC130001)
pEYFP-C1:pEYFP-C1; resistance: Kanamycin; Plasmid encoding the p21-binding domain (PBD) of human PAK1 was a gift from Klaus Hahn.: Plasmid encoding the p21-binding domain (PBD) of human PAK1 was a gift from Klaus Hahn.; created by: Joel A. Swanson
259. pCDH-EF1-MCS-T2A-Puro; resistance: AMP; created by: System_Biosciences
260. temp.
261. pLOX-CW-CRE; Insert:CRE pHR:pHR; resistance: Ampicillin; created by: Didier Trono
262. H2B-EGFP; Insert:H2B, Human histone & Neomycin (HIST1H2BJ, H2B/r, H2BFR )
EGFP-N1:EGFP-N1; resistance: KAN; created by: Geoff Wahl
263. pENTR1A-p16-IRES-EGFP; Insert:p16-ires-EGFP (p16, EGFP)
pENTR1A:pENTR1A; resistance: Kan; Addgene plasmid ? corrected for mutation (WonJae Lee): Addgene plasmid ? corrected for mutation (WonJae Lee); created by: WonJae_Lee